MDH1 抗体
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- 抗原 See all MDH1 抗体
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
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适用
- Pig
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This MDH1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
- Malate Dehydrogenase [Pig Heart].
- Top Product
- Discover our top product MDH1 Primary Antibody
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anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibody
MDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B11-B7 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (C-Term) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 265-334) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB 宿主: 小鼠 Monoclonal CPTC-MDH1-1 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 101-193) antibodyMDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 211-223) antibodyVerified MDH1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-334) antibodyMDH1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MalateDehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 80.0 mg/mL (by Refractometry)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
- 别名
- MDHA (MDH1 产品)
- 别名
- MDH-s antibody, MDHA antibody, MGC:1375 antibody, MOR2 antibody, ODS1 antibody, B230377B03Rik antibody, D17921 antibody, Mor-2 antibody, Mor2 antibody, MDL1 antibody, Mdhl antibody, MDH antibody, mdh-s antibody, mdha antibody, mor2 antibody, MDH-1 antibody, Mdh antibody, Mdh-1 antibody, Mdh2 antibody, MdhD antibody, cMdh antibody, sMdh antibody, malate dehydrogenase 1 antibody, malic enzyme 2 antibody, malate dehydrogenase 1, NAD (soluble) antibody, malate dehydrogenase 1 S homeolog antibody, malate dehydrogenase, cytoplasmic antibody, Malate dehydrogenase 1 antibody, MDH1 antibody, ME2 antibody, Mdh1 antibody, mdh1.S antibody, LOC100280767 antibody
- 背景
- Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic
- 基因ID
- 396894
- NCBI登录号
- NP_999039
- UniProt
- P11708
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