COL6 抗体 (Biotin)
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- 抗原 See all COL6 抗体
- COL6 (Collagen, Type VI (COL6))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL6 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule .
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Collagen type VI purified from Human and Bovine placenta.
- 亚型
- IgG
- Top Product
- Discover our top product COL6 Primary Antibody
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anti-Collagen, Type VI (COL6) antibody
COL6 适用: 人, Cow WB, ELISA, IHC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA, IHC, IHC (p), IHC (fro), ICC, DB 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibody (HRP)COL6 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal HRP
anti-Collagen, Type VI (COL6) antibody (FITC)COL6 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal FITC
anti-Collagen, Type VI (COL6) antibody (Biotin)COL6 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type VI (COL6) antibody (Biotin)COL6 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type VI (COL6) (AA 1-336) antibodyCOL6 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type VI (COL6) antibodyCOL6 适用: 人, 哺乳动物 WB, ELISA, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Collagen VI at a dilution of 1: 5,000 to 1: 10,000. This product was alsoassayed against 1.0 μg of Collagen VI in a standard sandwich ELISA using PeroxidaseConjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the stock concentration is suggested for this product. ForImmunohistochemistry on frozen tissue sections dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent)
- 浓度
- 1.0 mg/mL (by UV absorbance at 280)
- 缓冲液
- 0.125 M Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (Immunoglobulin and Protease free)
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.
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- 抗原
- COL6 (Collagen, Type VI (COL6))
- Abstract
- COL6 产品
- 背景
- The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.Synonyms: COL6A1
- 基因ID
- 1291
- NCBI登录号
- NP_001839
- UniProt
- P12109
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