RFX5 抗体 (N-Term)
-
- 抗原 See all RFX5 抗体
- RFX5 (Regulatory Factor X 5 (RFX5))
-
抗原表位
- N-Term
-
适用
- 人, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RFX5 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)
- 特异性
- Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa. Reacts with human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the N-terminal region. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.
- 纯化方法
- Prepared from monospecific antiserum by delipidation and defibrination.
- 免疫原
- RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Top Product
- Discover our top product RFX5 Primary Antibody
-
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibody
RFX5 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 32-0) antibodyRFX5 适用: 人 WB, GS 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 367-616) antibodyRFX5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 1-616) antibodyRFX5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (AA 516-616) antibodyRFX5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Regulatory Factor X 5 (RFX5) (Internal Region) antibodyVerified RFX5 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) antibodyRFX5 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (C-Term) antibodyRFX5 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Regulatory Factor X 5 (RFX5) (N-Term) antibodyRFX5 适用: 人, 犬, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Suitable for Immunoprecipitation, Western blotting (1/1000), ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates ata dilution of 1: 500 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (N-terminal specific) is suitable for the detection byimmunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gelsupershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 l perassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative., None
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.
-
- 抗原
- RFX5 (Regulatory Factor X 5 (RFX5))
- 别名
- RFX5 (RFX5 产品)
- 别名
- regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody
- 背景
- RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5
- 基因ID
- 5993
- NCBI登录号
- NP_000440
- UniProt
- P48382
-
