IL1A 抗体 (Biotin)
Quick Overview for IL1A 抗体 (Biotin) (ABIN116385)
抗原
See all IL1A 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (>98%) recombinant rIL-1 alpha
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anti-Interleukin 1 alpha (IL1A) (AA 172-271) antibody
IL1A 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4F9 unconjugated
anti-Interleukin 1 alpha (IL1A) antibodyIL1A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6G4A5 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21521 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 113-271) antibodyIL1A 适用: 人 WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 90-268) antibodyIL1A 适用: 山羊 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 112-271) antibodyIL1A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 115-270) antibodyIL1A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 113-271) antibodyIL1A 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 1 alpha (IL1A) antibodyIL1A 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2A2B3 unconjugated
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应用备注
- ELISA: (Direct): To detect Rat IL-1α by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat IL-1α. (Sandwich): To detect Rat IL-1α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Rat IL-1α (PP1133P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1α. Western blot: To detect Rat IL-1α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat IL-1α is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- Interleukin-1 alpha / IL-1A
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背景
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
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基因ID
- 24493
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NCBI登录号
- NP_058715
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UniProt
- P16598
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原
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