Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15.00 ng/mL), a concentration of 0.52-0.90 μg/mL of this antibody is required. ELISA: Indirect: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with compatible secondary reagents, it allows the detection of atleast 0.2 - 0.4 ng/well of recombinant mMIP-2. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with Biotinylated Anti-Murine MIP-2 (PP1082B) as a detectionantibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-2. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents thedetection limit for recombinant mMIP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.
GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2