IL1A 抗体
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北京 101111
Quick Overview for IL1A 抗体 (ABIN116063)
抗原
See all IL1A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Human Interleukin-1 alpha.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly purified (>98%) recombinant Interleukin 1 alpha.
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anti-Interleukin 1 alpha (IL1A) (AA 172-271) antibody
IL1A 适用: 人 WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 4F9 unconjugated
anti-Interleukin 1 alpha (IL1A) antibodyIL1A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6G4A5 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21521 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 113-271) antibodyIL1A 适用: 人 WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 112-271) antibodyIL1A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 90-268) antibodyIL1A 适用: 山羊 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 115-270) antibodyIL1A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 113-271) antibodyIL1A 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 1 alpha (IL1A) (N-Term) antibodyIL1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect hIL-1alpha by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-1 alpha. Western Blot: Use a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1alpha (0.1 ng/mL), a concentration of 0.01-0.01 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month, or (in aliquots) at -20 °C for longer.
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- Interleukin-1 alpha / IL-1A
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背景
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
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基因ID
- 3552
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NCBI登录号
- NP_000566
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UniProt
- P01583
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原
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