IGF1 抗体
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Quick Overview for IGF1 抗体 (ABIN116057)
抗原
See all IGF1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Insulin Like Growth Factor-1.
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交叉反应 （详细）
- Species reactivity (tested):Human
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纯化方法
- Affinity chromatography employing immobilized hIGF-1 matrix
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免疫原
- Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1)
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anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibody
IGF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-153) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (Internal Region) antibodyVerified IGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144), (C-Term) antibodyIGF1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB33711 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144) antibodyIGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-100) antibodyIGF1 适用: 人, 小鼠, 大鼠, 犬 WB, IP, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 33-102) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) antibody (Atto 488)IGF1 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIGF-1 (5.0 ng/mL), a concentration of 0.67 - 1.0 μg/mL of this antibody is required. ELISA: To detect hIGF-1 by ELISA (using 100 μL/well antibody solution) a concentrationof 0.5 - 2.0 μg/mL of this antibody is required. This allows the detection of 0.2 - 0.4 ng/wellof recombinant hIGF-1. Western blot: 0.1 - 0.2 μg/mL. The detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane,under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from date of despatch. Reconstituted antibody is stable for 2 weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 12 months
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- Insulin-Like Growth Factor I / IGF1
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背景
- Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, MGF, Mechano growth factor, Somatomedin-C
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基因ID
- 3479
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NCBI登录号
- NP_001104755
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UniProt
- P05019
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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