G-CSF 抗体
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北京 101111
Quick Overview for G-CSF 抗体 (ABIN116025)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Granulocyte Colony Stimulating Factor (G-CSF).
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography employing immobilized hG-CSF matrix.
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免疫原
- Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060)
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman G-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human G-CSF (PP1011B) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanG-CSF. Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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背景
- Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin
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基因ID
- 1440
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NCBI登录号
- NP_000750
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UniProt
- P09919
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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