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G-CSF 抗体

This anti-G-CSF antibody is a 兔 多克隆 antibody detecting G-CSF in WB, IHC (p), EIA 和 Func. Suitable for 人.
产品编号 ABIN116025
发货至: 中国

Quick Overview for G-CSF 抗体 (ABIN116025)

抗原

See all G-CSF (CSF3) 抗体
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

适用

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宿主

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克隆类型

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多克隆

标记

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This G-CSF antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    Recognizes Granulocyte Colony Stimulating Factor (G-CSF).

    交叉反应 (详细)

    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography employing immobilized hG-CSF matrix.

    免疫原

    Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060)
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman G-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human G-CSF (PP1011B) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanG-CSF. Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    别名

    G-CSF

    背景

    Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

    基因ID

    1440

    NCBI登录号

    NP_000750

    UniProt

    P09919

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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