G-CSF 抗体 (Biotin)
Quick Overview for G-CSF 抗体 (Biotin) (ABIN116023)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Granulocyte Colony Stimulating Factor (G-CSF).
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纯化方法
- Affinity Chromatography employing immobilized hG-CSF matrix.
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免疫原
- Highly pure (>98%) recombinant hG-CSF (human G-CSF)
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应用备注
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ELISA: (Direct): To detect hG-CSF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hG-CSF. (Sandwich): To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human G-CSF (PP1011P1 or PP1011P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C
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储存方法
- Store the antibody at 2-8 °C. DO NOT FREEZE!
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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背景
- Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin
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基因ID
- 1440
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NCBI登录号
- NP_000750
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UniProt
- P09919
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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