G-CSF 抗体
Quick Overview for G-CSF 抗体 (ABIN115925)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognises Murine G-CSF.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant mG-CSF
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anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 155-198) antibody
CSF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8G5F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7E4F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 30-207) antibodyCSF3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 小鼠 Monoclonal rCSF3-900 unconjugated Recombinant Antibody
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 47-205) antibodyCSF3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 27-200) antibodyCSF3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 兔 Monoclonal CSF3-3166R unconjugated Recombinant Antibody
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mG-CSF (0.5 ng/mL),a concentration of 0.005-0.010 μg/mL of this antibody is required. ELISA: (Indirect): To detect mG-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mG-CSF. (Sandwich): To detect mG-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine G-CSF (PP022B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF. Western Blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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背景
- Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin
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基因ID
- 12985
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NCBI登录号
- NP_034101
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UniProt
- P09920
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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