TNF alpha 抗体
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北京 101111
Quick Overview for TNF alpha 抗体 (ABIN115913)
抗原
See all TNF alpha 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography employing immobilized MouseTNF-alpha matrix
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免疫原
- Highly pure (>98%) E.coli derived recombinant Mouse TNF-alpha
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 大鼠 IHC, FACS, IF 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha 适用: 人, Pig, Non-Human Primate ICFC 宿主: 小鼠 Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 201-233), (C-Term) antibodyTNF alpha 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 71-234) antibodyTNF alpha 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmTNF-alpha (0.5 ng/mL), a concentration of 0.010 - 0.015 μg/mL of this antibody isrequired. ELISA (Indirect): To detect mTNF-alpha by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with compatible secondary reagents, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant mTNF-alpha. ELISA (Sandwich): To detect mTNF-alpha by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with a compatible biotinylated secondaryantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-alpha. Western Blot: To detect mTNF-alpha by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mTNF-alpha is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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别名
- Tumor Necrosis Factor (TNF-Alpha)
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背景
- Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
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基因ID
- 10090
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UniProt
- P06804
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途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
抗原
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