TNF alpha 抗体
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- 抗原 See all TNF alpha 抗体
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNF alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Recombinant Human TNF-alpha.
- 亚型
- IgG1
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman TNF-alpha (0.5 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human TNF-alpha when used withBiotin anti-Human TNF-alpha antibody (cat. PP1071B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sectionsof human breast invasive ductal carcinoma. A concentarion of 15.0-20.0 μg/mL isrecommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
- 缓冲液
- PBS without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- Tumor Necrosis Factor (TNF-Alpha) (TNF alpha 产品)
- 别名
- DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody
- 背景
- Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
- 基因ID
- 9606
- UniProt
- P01375
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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