RAF1 抗体
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北京 101111
Quick Overview for RAF1 抗体 (ABIN114553)
抗原
See all RAF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts to Raf-1 N-terminal (1-240 a.a.)
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纯化方法
- Protein G affinity purified
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免疫原
- Purified recombinant Raf-1 (mw:75 kDa)
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亚型
- IgG1
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应用备注
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ELISA. Western Blot: An antibody concentration of 1 μg/mL will allow visualization of 0.2 g/laneof human Raf-1. A main band (75 kDa) was visible.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/mL
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缓冲液
- 0.01 M PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
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- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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别名
- RAF1
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背景
- The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
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基因ID
- 5894
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NCBI登录号
- NP_002871
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UniProt
- P04049
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途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
抗原
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