Collagen Type I 抗体
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Quick Overview for Collagen Type I 抗体 (ABIN113983)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Collagen type I: 100 % Collagen type II and IV: < 0.1 % Collagen type III and V: < 1.0 % Human Fibronectin: < 0.1 %.
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交叉反应 （详细）
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Species reactivity (expected):Cattle (< 1.0 %), Rat and Chicken (< 0.1 %).
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography
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免疫原
- Purified Collagen type I from Human skin.
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 小鼠, 大鼠, 兔, 鱼, 犬 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), ICC, IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4H10 unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian WB, IHC (fro), IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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RIA. ELISA: >/= 1/200 (OD >/= 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Human skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered solution, no BSA and preservative added.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
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基因ID
- 1277
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NCBI登录号
- NP_000079
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UniProt
- P02452
抗原
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