S100A8/A9 Complex (Calprotectin) 抗体
S100A8/A9
适用: 人, Cow, Pig
IHC (p), EIA, IHC (fro)
宿主: 小鸡
Polyclonal
unconjugated
-
- 抗原 See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
-
适用
- 人, Cow, Pig
-
宿主
-
小鸡
-
克隆类型
- 多克隆
-
标记
- This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated
-
应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This polyclonal antibody recognizes different epitopes on the MRP8/14 heterocomplex (S100A8/S100A9). It is particularly suited for use in ELISA or similar assays. It was generated by immunising chickens with recombinant MRP8/14. Thus, the antibody does not discriminate between the individual subunits MRP8 or MRP14. In Immunohistochemistry, it detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears. The MRP8/14 specific ELISA has shown significant advantages in the early assessment of certain acute inflammatory conditions. Antigen Distribution on Tissue Sections: Macrophages in the red pulp of the spleen and in the liver, strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen, absent on normal resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).
- 交叉反应 （详细）
-
Species reactivity (expected):Bovine and Swine.
Species reactivity (tested):Human. - 纯化方法
- Affinitty Chromatography.
- 免疫原
- Recombinant MRP8 and MRP14 Remarks: The antigen is produced by the Ca2+-dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
- Top Product
- Discover our top product S100A8/A9 Primary Antibody
-
-
- 应用备注
-
ELISA. Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/2000). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/250). Proteinase K pretreatmentfor antigen retrieval is recommended. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
- 别名
- MRP8 / MRP14 (S100A8/A9 产品)
- 别名
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, S100 calcium binding protein A9 antibody, S100A9 antibody
- 背景
- Synonyms: CAGA, CAGB, CFAG, Calgranulin A/B, Calprotectin, L1 Protein, MRP-14, MRP-8, P14, P8
- 途径
- S100 Proteins
-
You are here:
