Western Blot: reacts with a band at 200-220 kDa in reducing gels of newborn rat or mousecell extracts, For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and4.6 % SDS, Membranes should be blocked with milk or BSA, 5 % PAGE gels are suggested. Immunohistochemistry on frozen and paraffin sections: 1/20 - 1/200, We recommendperfusing tissues with 4 % paraformaldehyde at pH 7.4 for light microscopy or with either4 % paraformaldehyde at pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at pH 7.4 for EM, For Immunocytochemistry we recommend using cells fixed in 4 %paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
TRIS buffered saline, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
背景
Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.Synonyms: NES, Nbla00170