SERPINA3 抗体
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- 抗原 See all SERPINA3 抗体
- SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SERPINA3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes alpha-1 Antichymotrypsin in paraffin embedded and frozen Human cells and tissues. Monocytes and macrophages are recognized in tonsils.
- 纯化方法
- Affinity Chromatography
- 克隆位点
- ACT14C7
- 亚型
- IgG1
- Top Product
- Discover our top product SERPINA3 Primary Antibody
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anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 1-423) antibody
SERPINA3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 279-432) antibodySERPINA3 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5G3C11 unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 25-423) antibodySERPINA3 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 23-253) antibodySERPINA3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 25-423) antibodySERPINA3 适用: 人 ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 24-423) antibodySERPINA3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 235-394) antibodySERPINA3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) antibodySERPINA3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 1-423) antibodySERPINA3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3) (AA 121-320) antibodySERPINA3 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400). Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/400), Microwave pretreatment forantigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS buffer pH 7.2 containing 0.1 % Kathon as preservative and 5 mg/mL BSA as stabilizer
- 储存液
- Kathon CG
- 注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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- 抗原
- SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))
- 别名
- alpha-1-Antichymotrypsin (ACT) (SERPINA3 产品)
- 别名
- SERPINA3-1 antibody, Pi2 antibody, AACT antibody, ACT antibody, GIG25 antibody, A1AT antibody, PI antibody, alpha-1-PI antibody, AAT antibody, Pi antibody, Spi1 antibody, SERPINA3-5 antibody, serpina3 antibody, serpin A3-5 antibody, serpin family A member 3 antibody, alpha-1-antiproteinase F precursor antibody, serpin family A member 1 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog antibody, LOC396684 antibody, SERPINA3 antibody, LOC100008973 antibody, SERPINA1 antibody, Serpina1 antibody, SPIA5 antibody, serpina3.L antibody
- 背景
- The protein is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variation in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.Synonyms: AACT, Cell growth-inhibiting gene 24/25 protein, GIG24, GIG25, SERPINA3
- 基因ID
- 12
- NCBI登录号
- NP_001076
- UniProt
- P01011
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