Transferrin Receptor 抗体
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- 抗原 See all Transferrin Receptor (TFRC) 抗体
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Transferrin Receptor antibody is un-conjugated
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应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography.
- 免疫原
- KG-1 cells
- 克隆位点
- VIP-1
- 亚型
- IgG1
- Top Product
- Discover our top product TFRC Primary Antibody
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anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibodyTFRC 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibodyTFRC 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibodyTFRC 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibodyTFRC 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (Biotin)TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 Biotin
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibodyTFRC 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 1A1B2 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 621-720) antibodyTFRC 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 567-744) antibodyTFRC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 572-757) antibodyTFRC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water (= 0.2 mg/mL stock solution).
- 浓度
- 0.2 mg/mL
- 缓冲液
- Stock solution contains PBS, pH 7.2 with 0.02 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- All human source materials have tested negative for HIV1, HIV2, HBc, HCV and HBsAg antibodies. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- RT/4 °C/-20 °C
- 储存方法
- Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.
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- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- CD71 / TFRC (TFRC 产品)
- 别名
- Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody
- 背景
- CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane.Synonyms: TfR1, Transferrin receptor protein 1, p90
- 基因ID
- 9606
- UniProt
- P02786
- 途径
- Transition Metal Ion Homeostasis
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