FCGR2A 抗体
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- 抗原 See all FCGR2A 抗体
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FCGR2A antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This Monoclonal Antibody 2E1 recognizes the three isoforms of the Fc-gamma RII designated as A, B and C. Monocytes carry all forms of Fc-gamma RII, Neutrophils carry Fc-gamma RIIA and Fc-gamma RIIC. Fc-gamma RIIB is expressed by B lymphocytes and mast cells.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography
- 免疫原
- K562 cells.
- 克隆位点
- 2-00E-001
- 亚型
- IgG2a
- Top Product
- Discover our top product FCGR2A Primary Antibody
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anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibody
FCGR2A 适用: 人 WB, IF, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 9C6 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A 适用: 人, 猴 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 20D1 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 241-317) antibodyFCGR2A 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (C-Term) antibodyFCGR2A 适用: 人 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A 适用: 人 WB, IF, IHC, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal 6H7 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 34-316) antibodyFCGR2A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 1-316) antibodyFCGR2A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (AA 46-150) antibodyFCGR2A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E8 unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) (C-Term) antibodyFCGR2A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibodyFCGR2A 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 3B6 unconjugated Recombinant Antibody
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- 应用备注
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FACS: 10 μL stock solution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 2 μg/mL (1/200). ELISA. Immunoprecipitation. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water (stock solution contains 0.4 mg/mL IgG)
- 浓度
- 0.4 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2-8 °C before opening. DO NOT FREEZE! This product is stable at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, add glycerol to a final concentration of 50% and store at -20 °C. Freezing alkaline phosphatase conjugates without glycerol will result in a substantial loss of enzymatic activity.
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- 抗原
- FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
- 别名
- CD32 / FcRII-A (FCGR2A 产品)
- 别名
- CD32 antibody, CD32A antibody, CDw32 antibody, FCG2 antibody, FCGR2 antibody, FCGR2A1 antibody, FcGR antibody, IGFR2 antibody, CD32a antibody, Fc fragment of IgG receptor IIa antibody, FCGR2A antibody
- 背景
- CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
- 基因ID
- 2212
- NCBI登录号
- NP_001129691
- UniProt
- P12318
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