CD14 抗体
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北京 101111
Quick Overview for CD14 抗体 (ABIN111907)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14. BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized.
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纯化方法
- Affinity Chromatography.
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免疫原
- Human Monocytes.
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亚型
- IgG2b
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anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated
anti-CD14 (CD14) (AA 20-214) antibodyCD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated
anti-CD14 (CD14) (AA 20-345) antibodyCD14 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 14G1F3 unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated
anti-CD14 (CD14) (AA 230-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated
anti-CD14 (CD14) (AA 54-83), (N-Term) antibodyCD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated
anti-CD14 (CD14) (AA 201-300) antibodyCD14 适用: 人, 小鼠, 大鼠, 绵羊, 犬, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (Internal Region) antibodyVerified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-CD14 (CD14) (AA 25-148) antibodyCD14 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2397 unconjugated
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应用备注
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Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Does not react on routinely processed Paraffin Sections. Recommended Positive Control: Human placenta. Has been described to work in FACS (post fixation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL distilled water (= Stock solution).
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浓度
- 0.2 mg/mL
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缓冲液
- PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- This product is photosensitive and should be protected from light
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!
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- CD14
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别名
- CD14
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背景
- CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
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基因ID
- 929, 9606
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UniProt
- P08571
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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