CD45RB 抗体 (APC)
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- 抗原 See all CD45RB 抗体
- CD45RB
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45RB antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Mouse monoclonal Anti-Human CD45RB APC is recommended for use in flow cytometry.
- 克隆位点
- MC5-2
- 亚型
- IgG1
- Top Product
- Discover our top product CD45RB Primary Antibody
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anti-CD45RB (CD45RB) antibody
CD45RB 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-55 unconjugated
anti-CD45RB (CD45RB) antibody (FITC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 FITC
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 PE
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-55 APC
anti-CD45RB (CD45RB) antibody (PE)CD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A PE
anti-CD45RB (CD45RB) (AA 79-88) antibodyCD45RB 适用: 人 FACS, IHC, IHC (p) 宿主: 小鼠 Monoclonal MEM-143 unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal C363-16A unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 人, 猴, 猕猴, 兔 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM55 unconjugated
anti-CD45RB (CD45RB) antibodyCD45RB 适用: 小鼠 FACS, IHC (p), IP, IHC (fro) 宿主: 大鼠 Monoclonal unconjugated
anti-CD45RB (CD45RB) antibody (APC)CD45RB 适用: 人, 猴, 猕猴 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-55 APC
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 样品制备
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- Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD45RB APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype 4. Incubate in the dark at room temperature at 4°C for 15 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.5 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD45RB
- 别名
- CD45RB (CD45RB 产品)
- 别名
- B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody
- 背景
- The antibody reacts with the LCA complex expressed on all haemopoietic cells but not platelets (190, 205, 200 kDa).
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