电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Collagen Type I 抗体

COL1 适用: 人 IHC (fro), EIA 宿主: 小鼠 Monoclonal BDI314 unconjugated
产品编号 ABIN111171
发货至: 中国
  • 抗原 See all Collagen Type I (COL1) 抗体
    Collagen Type I (COL1) (Collagen, Type I (COL1))
    适用
    • 96
    • 63
    • 59
    • 20
    • 18
    • 17
    • 15
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    宿主
    • 67
    • 35
    • 14
    小鼠
    克隆类型
    • 81
    • 35
    单克隆
    标记
    • 56
    • 14
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    This Collagen Type I antibody is un-conjugated
    应用范围
    • 71
    • 46
    • 38
    • 34
    • 32
    • 27
    • 27
    • 26
    • 19
    • 18
    • 16
    • 8
    • 6
    • 5
    • 5
    • 4
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    Both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and II in ELISA, RIA or Immunoblotting.
    纯化方法
    Ammonium sulfate precipitation and chromatography on DEAE-cellulose
    克隆位点
    BDI314
    亚型
    IgG
    Top Product
    Discover our top product COL1 Primary Antibody
  • 应用备注
    ELISA: Apparent binding constant from titration curves is approximately 0.1 nM. Antibodysuitable for use in sandwich ELISA for specific determination of collagen I, employed aseither a capture antibody or as a second, detecting antibody. Immunohistochemical staining of 10 m thick cryostat sections fixed with acetone orethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is notrecommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 1 mL distilled water.
    储存液
    Azide free
    储存条件
    -20 °C
    储存方法
    Store at -20 °C. Avoid multiple freeze/thaw cycles.
  • 抗原
    Collagen Type I (COL1) (Collagen, Type I (COL1))
    别名
    Collagen Type I (COL1 产品)
    别名
    collagen type III alpha 1 chain antibody, COL3A1 antibody
    背景
    Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
    基因ID
    1277
    NCBI登录号
    NP_000079
    UniProt
    P02452
You are here:
客服