Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN111171)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and II in ELISA, RIA or Immunoblotting.
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纯化方法
- Ammonium sulfate precipitation and chromatography on DEAE-cellulose
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亚型
- IgG
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), ICC, IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian WB, IHC (fro), IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA, FLISA 宿主: 小鼠 Monoclonal 4F6 Biotin
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应用备注
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ELISA: Apparent binding constant from titration curves is approximately 0.1 nM. Antibodysuitable for use in sandwich ELISA for specific determination of collagen I, employed aseither a capture antibody or as a second, detecting antibody. Immunohistochemical staining of 10 m thick cryostat sections fixed with acetone orethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is notrecommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL distilled water.
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Avoid multiple freeze/thaw cycles.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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背景
- Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
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基因ID
- 1277
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NCBI登录号
- NP_000079
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UniProt
- P02452
抗原
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