(1 reference)-
- 抗原
- mEOS2
- 适用
- Lobophyllia hemprichii
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- ELISA
- 特异性
- This antibody binds specifically to mEos2 GFP.
- 纯化方法
- Affinity Purified
- 纯度
- The IgG fraction of tissue culture supernatant was purified by Protein A / G affinity chromatography.
- 免疫原
- The immunogen was recombinant mEos2 GFP. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen.
- 克隆位点
- 12C11-C8
- 亚型
- IgG2a
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- 应用备注
- For ELISA we recommend a work dilution of 1: 64,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the antibody with distilled or de-ionized water to a final concentration of 1 mg/ml.
- 缓冲液
- Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives.
- 储存方法
- Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
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: "Rational design of true monomeric and bright photoactivatable fluorescent proteins." in: Nature methods, Vol. 9, Issue 7, pp. 727-9, (2012) (PubMed).
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: "Rational design of true monomeric and bright photoactivatable fluorescent proteins." in: Nature methods, Vol. 9, Issue 7, pp. 727-9, (2012) (PubMed).
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- 抗原
- mEOS2
- 背景
- Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for superresolution microscopy. An optical highlighter isolated from another stony coral (Lobophyllia hemprichii), the tetrameric EosFP (named after the goddess of dawn in Greek mythology), behaves similarly to Kaede and the other variants described above. EosFP emits bright green fluorescence at 516 nanometers, and can be photoconverted to orange-red (581 nanometers) fluorescence when illuminated at near-ultraviolet wavelengths. Random and site-directed mutagenesis of tetrameric EosFP was used to generate two dimers and a true monomeric protein named mEosFP.
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