Muscarinic Acetylcholine Receptor M2 抗体 (Extracellular Loop)
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- 抗原 See all Muscarinic Acetylcholine Receptor M2 (CHRM2) 抗体
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
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抗原表位
- AA 168-192, Extracellular Loop
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适用
- 人, 小鼠, 大鼠, 豚鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 序列
- VRTVEDGECY IQFFSNAAVT FGTAI(C)
- 特异性
- The monoclonal antibody B8E5 recognizes Human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the Human M2 receptor.
- 交叉反应 (详细)
- Species reactivity (tested):Human, Guinea pig, Mouse, Rat.
- 纯化方法
- Protein G Chromatography
- 免疫原
- Human second extracellular loop residues 168-192
- 克隆位点
- B8E5
- 亚型
- IgG2a
- Top Product
- Discover our top product CHRM2 Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.1 % BSA
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 2-8 °C.
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- 抗原
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
- 别名
- Muscarinic Acetylcholine Receptor M2 (CHRM2 产品)
- 别名
- HM2 antibody, AChR-M2 antibody, Chrm-2 antibody, M2 antibody, chrm2 antibody, Acm2 antibody, GPM2 antibody, cholinergic receptor muscarinic 2 antibody, cholinergic receptor, muscarinic 2, cardiac antibody, cholinergic receptor, muscarinic 2a antibody, cholinergic receptor, muscarinic 2 antibody, CHRM2 antibody, Chrm2 antibody, chrm2a antibody
- 背景
- This receptor is an integral membrane protein consisting of seven membrane spanning a-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.Synonyms: CHRM2, mAChR M2, mAChR-M2
- 基因ID
- 1129
- NCBI登录号
- NP_000730
- UniProt
- P08172
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