Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN1106740)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Specificity was ascertained by direct ELISA using pure Dog Collagen types I and III for coating microplate wells. No binding to canine serum proteins is revealed at simular dilutions of the antibody. Characteristic immunostaining pictures of frozen sections of dog kidney and skin are produced to certify absence of crossreactivity with basement membrane collagens (types IV and V).
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交叉反应 （详细）
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Species reactivity (tested):Canine (Dog).
Also reacts to: type III -
纯化方法
- Affinity Chromatogaryphy: The affinity purified antibody is obtained by binding to immobilized native dog collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
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免疫原
- Native Dog Collagen, mixture of type I and III: Antibodies to Dog Collagen type I and III are raised in rabbits which are numerously immunized with extensively purified native collagen type I and III extracted from dog tail tendon into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction.
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亚型
- IgG
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), ICC, IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian WB, IHC (fro), IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA, FLISA 宿主: 小鼠 Monoclonal 4F6 Biotin
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 1 mL distilled water and add preservative if preferred
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浓度
- 0.1 mg/mL
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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背景
- It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
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基因ID
- 403651
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NCBI登录号
- NP_001003090
抗原
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