This Monoclonal Antibody ED1 is useful for detecting Rat monocytes and macrophages and isolated dendritic (veiled) cells in the blood. The antibody recognises a single chain glycoprotein of 90-100 kDa. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 has many characteristics in common with Mouse macrosialin and Human CD68. Antigen, Epitope: CD68, ED1 recognises a 92 kDa cytoplasmic protein. The epitope has not been further characterized. Antigen Distribution: The antigen is found on 90 % of monocytes in the peripheral blood. It is also expressed by 98 % of isolated dendritic (veiled) cells.
交叉反应 (详细)
Species reactivity (tested):Rat: Monocytes Macrophages, Dendritic Cells.
The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin