PCNA 抗体 (AA 111-122)
Quick Overview for PCNA 抗体 (AA 111-122) (ABIN1049498)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 111-122
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原理
- PCNA (aa111-122)
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序列
- EAPNQEKVSD YE
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特异性
- Reported variants represent identical protein: NP_872590.1, NP_002583.1
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EAPNQEKVSDYE, from the internal region of the protein sequence according to NP_002583.1.
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亚型
- IgG
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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibodyPCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibodyPCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibodyPCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H10 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8 kDa according to Human NP_002583.1). Approx.35 kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33 kDa in Pig Spleen
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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别名
- PCNA
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背景
- PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin
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基因ID
- 5111, 18538, 25737
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NCBI登录号
- NP_002583
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
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