TRPM8 抗体 (Internal Region)
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- 抗原 See all TRPM8 抗体
- TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))
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抗原表位
- Internal Region
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适用
- 人, 大鼠, 小鼠, 豚鼠, 犬, 兔, 马, Bat, Hamster, 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TRPM8 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human TRPM8. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRPM2 (60 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Rabbit, Horse, Guinea pig, Turkey, Chicken, Armadillo, Platypus (100%) Galago, Marmoset, Bovine, Pig, Opossum, Xenopus (93%) Lizard (87%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from internal region of human TRPM8. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Rabbit, Horse, Guinea pig, Turkey, Chicken, Armadillo, Platypus (100%), Galago, Marmoset, Bovine, Pig, Opossum, Xenopus (93%), Lizard (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TRPM8 Primary Antibody
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anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 926-956), (C-Term) antibody
TRPM8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14420 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 521-552) antibodyTRPM8 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 263-292) antibodyTRPM8 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (3rd Extracellular Loop), (AA 917-929) antibodyTRPM8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, IP, LCI, Neut 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 1-192) antibodyTRPM8 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (N-Term) antibodyTRPM8 适用: 人, 豚鼠, 犬, 兔, Bat, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 933-963) antibodyTRPM8 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 980-1104) antibodyTRPM8 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (Internal Region) antibodyTRPM8 适用: 人, 大鼠, 小鼠, 豚鼠, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (AA 251-300) antibodyTRPM8 适用: 人, 豚鼠, 犬, 兔, 马, Cow, Pig, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2.4 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))
- 别名
- TRPM8 (TRPM8 产品)
- 别名
- TRPM8 antibody, trpp8 antibody, ltrpc6 antibody, trpm8b antibody, LTRPC6 antibody, TRPP8 antibody, CMR1 antibody, LTrpC-6 antibody, Trp-p8 antibody, transient receptor potential cation channel subfamily M member 8 antibody, transient receptor potential cation channel subfamily M member 8b L homeolog antibody, transient receptor potential cation channel, subfamily M, member 8 antibody, TRPM8 antibody, trpm8b.L antibody, Trpm8 antibody
- 背景
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Name/Gene ID: TRPM8
Subfamily: Calcium channel - TRP
Family: Ion Channel
Synonyms: TRPM8, CMR1, Cold-menthol receptor type 1, LTrpC-6, TRPP8, Trp-p8, LTRPC6 - 基因ID
- 79054
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