TRPA1 抗体 (Internal Region)
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- 抗原 See all TRPA1 抗体
- TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TRPA1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- IHC-plus™
- 特异性
- Human TRPA1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Marmoset, Bovine (89%) Monkey, Elephant, Dog, Rabbit, Pig (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from internal region of human TRPA1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Marmoset, Bovine (89%), Monkey, Elephant, Dog, Rabbit, Pig (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TRPA1 Primary Antibody
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- 应用备注
- Approved: IF, IHC, IHC-Fr, IHC-P (7.5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TRPA1 (Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))
- 别名
- TRPA1 (TRPA1 产品)
- 别名
- trpa1 antibody, ANKTM1 antibody, Anktm1 antibody, CG5751 antibody, CG5761 antibody, CT18073 antibody, DmTRPA1 antibody, Dmel\\CG5751 antibody, TRPA antibody, TRPA1 antibody, TrpA antibody, dANKTM1 antibody, dTRPA antibody, dTRPA1 antibody, dTrpA antibody, dTrpA1 antibody, dtrpA1 antibody, trpA1 antibody, GLEAN_02449 antibody, FEPS antibody, transient receptor potential cation channel, subfamily A, member 1a antibody, Transient receptor potential cation channel A1 antibody, transient receptor potential cation channel subfamily A member 1 antibody, transient receptor potential A1 antibody, tryptophan synthase subunit alpha 1 antibody, transient receptor potential cation channel, subfamily A, member 1 antibody, TRPA cation channel homolog antibody, trpa1a antibody, TrpA1 antibody, TRPA1 antibody, LOC658860 antibody, LOC694623 antibody, trpA1 antibody, trpa1 antibody, Trpa1 antibody, trpa-1 antibody
- 背景
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Name/Gene ID: TRPA1
Subfamily: Calcium channel - TRP
Family: Ion Channel
Synonyms: TRPA1, ANKTM1, TRPA-1 - 基因ID
- 8989
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