SGK3 抗体 (Internal Region)
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- 抗原 See all SGK3 抗体
- SGK3 (serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, 犬, Bat, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SGK3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human SGK3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Panda, Dog, Bat (100%) Monkey, Marmoset, Elephant, Bovine, Horse, Rabbit (95%) Turkey, Chicken, Platypus (90%) Opossum, Lizard (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from internal region of human SGK3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Panda, Dog, Bat (100%), Monkey, Marmoset, Elephant, Bovine, Horse, Rabbit (95%), Turkey, Chicken, Platypus (90%), Opossum, Lizard (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SGK3 Primary Antibody
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anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 1-30), (N-Term) antibody
SGK3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB0888 unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 40-90) antibodySGK3 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 1-130) antibodySGK3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A7 unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 141-368) antibodySGK3 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (N-Term) antibodySGK3 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (C-Term) antibodySGK3 适用: 人, 小鼠, 大鼠, Cow, 兔, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 107-156) antibodySGK3 适用: 人, Cow, 犬, 兔, 豚鼠, 马, 小鸡, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 108-139) antibodySGK3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1190 unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 381-409), (C-Term) antibodySGK3 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3) (AA 1-150) antibodySGK3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (1 - 36 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SGK3 (serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3))
- 别名
- SGK3 (SGK3 产品)
- 别名
- CISK antibody, SGK2 antibody, SGKL antibody, 2510015P22Rik antibody, A330005P07Rik antibody, Cisk antibody, fy antibody, fz antibody, Sgkl antibody, zgc:162253 antibody, serum/glucocorticoid regulated kinase family member 3 antibody, serum/glucocorticoid regulated kinase 3 antibody, serum/glucocorticoid regulated kinase family, member 3 antibody, serine/threonine-protein kinase Sgk3 antibody, SGK3 antibody, Sgk3 antibody, LOC464217 antibody, sgk3 antibody, LOC100231693 antibody, LOC100343709 antibody, LOC100052030 antibody, LOC100521447 antibody
- 背景
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Name/Gene ID: SGK3
Subfamily: SGK
Family: Protein Kinase
Synonyms: SGK3, CISK, SGKL - 基因ID
- 23678
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