P2RY8 抗体 (C-Term)
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- 抗原 See all P2RY8 抗体
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This P2RY8 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human P2RY8 / P2Y8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla (95%).
- 纯化方法
- Immunoaffinity Chromatography
- 免疫原
- Synthetic 20 amino acid peptide from C-terminus of human P2RY8 / P2Y8.
- Top Product
- Discover our top product P2RY8 Primary Antibody
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- 说明
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This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 浓度
- Lot specific
- 缓冲液
- PBS, 0.1% sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-80 °C
- 储存方法
- Long term: -70°C, Short term: +4°C
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- 抗原
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
- 别名
- P2RY8 / P2Y8 (P2RY8 产品)
- 别名
- P2Y8 antibody, P2Y purinoceptor 8 antibody, P2Y receptor family member 8 antibody, purinergic receptor P2Y8 antibody, p2ry8 antibody, P2RY8 antibody
- 背景
- Standard Gene Symbol: P2RY8, Gene Family: GPCR, Gene Subfamily: Orphan-A, Synonyms: P2RY8, p2RY8, p2Y purinoceptor 8, p2Y8
- 基因ID
- 286530
- UniProt
- Q86VZ1
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