P2RY12 抗体 (N-Term)
Quick Overview for P2RY12 抗体 (N-Term) (ABIN1049207)
抗原
See all P2RY12 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
品牌
- IHC-plus™
-
特异性
- Human P2RY12 / P2Y12. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
-
预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (94%) Gibbon, Marmoset (88%) Pig (82%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic 17 amino acid peptide from N-terminal extracellular domain of human P2RY12 / P2Y12. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey (94%), Gibbon, Marmoset (88%), Pig (82%).
Type of Immunogen: Synthetic peptide
-
-
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) antibody
P2RY12 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1C2A9 unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (AA 141-240) antibodyP2RY12 适用: 人, 小鼠 WB, ELISA, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) antibodyP2RY12 适用: 小鼠 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) antibodyP2RY12 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (AA 270-282), (Extracellular) antibodyP2RY12 适用: 人 WB, IHC, IF, LCI, IC 宿主: 豚鼠 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (Extracellular Domain) antibodyP2RY12 适用: 人, 犬, 猴, 马, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (N-Term) antibodyP2RY12 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (AA 125-142), (Intracellular) antibodyP2RY12 适用: 人 WB, IP, IHC, IF, FACS, IC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (AA 270-282), (Extracellular) antibodyP2RY12 适用: 人 WB, IF, FACS, LCI, IC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12) (AA 270-282), (Extracellular) antibody (Brilliant Violet 421™,Pacific Blue)P2RY12 适用: 人 FACS, LCI 宿主: 兔 Polyclonal Brilliant Violet 421™,Pacific Blue
-
-
应用备注
- Approved: ICC, IHC, IHC-P (32 μg/mL)
-
说明
-
Target Species of Antibody: Human
-
实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, less than 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
-
有效期
- 12 months
-
-
- P2RY12 (Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12))
-
别名
- P2RY12 / P2Y12
-
背景
-
Name/Gene ID: P2RY12
Subfamily: Purinergic
Family: GPCR
Synonyms: P2RY12, ADP-glucose receptor, ADPG-R, p2Y(12)R, p2Y(ADP), p2T(AC), p2Y12, Platelet ADP receptor, SP1999, p2RY12, p2Y purinoceptor 12, Purinergic receptor P2RY12, BDPLT8, Gi-coupled ADP receptor HORK3, HORK3, p2Y(AC), p2Y(cyc), p2Y12 platelet ADP receptor -
基因ID
- 64805
抗原
-
