NR5A2 + LRH1 抗体 (C-Term)
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- 抗原 See all NR5A2 + LRH1 (NR5A2) 抗体
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
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抗原表位
- C-Term
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适用
- 人, Cow, 绵羊, 犬, 马, Pig, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NR5A2 + LRH1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%) Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from C-terminal to ligand-binding domain of human NR5A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%), Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR5A2 Primary Antibody
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anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 1-140) antibody
NR5A2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 182-291) antibodyNR5A2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 206-495) antibodyNR5A2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) antibodyNR5A2 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (Internal Region) antibodyNR5A2 适用: 人, 大鼠, 小鼠, 犬, 马, 斑马鱼, 猴, 小鸡, Hamster, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (Internal Region) antibodyVerified NR5A2 适用: 人, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 328-338) antibodyVerified NR5A2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 1-205) antibodyNR5A2 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (AA 200-233) antibodyNR5A2 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal RB51100 unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2) (C-Term) antibodyNR5A2 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, 绵羊, 犬, 马, 斑马鱼, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (7 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))
- 别名
- NR5A2 / LRH-1 (NR5A2 产品)
- 别名
- xff1rashort antibody, NR5A2 antibody, b1f antibody, b1f2 antibody, cpf antibody, ff1r antibody, ftf antibody, ftz-f1 antibody, ftz-f1beta antibody, hb1f antibody, hb1f-2 antibody, lrh-1 antibody, lrh1 antibody, xff1ra antibody, B1F antibody, B1F2 antibody, CPF antibody, FTF antibody, FTZ-F1 antibody, FTZ-F1beta antibody, LRH-1 antibody, LRH1 antibody, hB1F-2 antibody, xFF1rB antibody, AU020803 antibody, D1Ertd308e antibody, Ftf antibody, UF2-H3B antibody, Lrh-1 antibody, nuclear receptor subfamily 5 group A member 2 S homeolog antibody, nuclear receptor subfamily 5 group A member 2 antibody, nuclear receptor subfamily 5 group A member 2 L homeolog antibody, nuclear receptor subfamily 5, group A, member 2 antibody, nr5a2.S antibody, NR5A2 antibody, nr5a2 antibody, nr5a2.L antibody, Nr5a2 antibody
- 背景
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Name/Gene ID: NR5A2
Subfamily: NR5 Fushi tarazu-like
Family: NHR
Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr - 基因ID
- 2494
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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