NR5A1 抗体 (Internal Region)
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- 抗原 See all NR5A1 抗体
- NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))
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抗原表位
- Internal Region
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适用
- 人, 猴, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NR5A1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIM23 (47 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%) Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%) Mouse, Rat, Hamster (88%) Turkey, Chicken, Platypus, Xenopus (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from internal region of human NR5A1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%), Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%), Mouse, Rat, Hamster (88%), Turkey, Chicken, Platypus, Xenopus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR5A1 Primary Antibody
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anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (Middle Region) antibody
NR5A1 适用: 人, 小鼠, 大鼠, 豚鼠, 犬, 马, 兔, Cow, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 172-221) antibodyNR5A1 适用: 人, 兔, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 1-120) antibodyNR5A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 64-93), (N-Term) antibodyNR5A1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB26159 unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (Middle Region) antibodyNR5A1 适用: 人, 大鼠, 豚鼠, 犬, 马, 兔, Cow, 绵羊, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) antibodyNR5A1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 174-223) antibodyNR5A1 适用: 人, 小鼠, 大鼠, 豚鼠, 马, Cow, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 358-407) antibodyNR5A1 适用: 小鼠, 大鼠, 豚鼠, 犬, 马, 兔, Pig, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 169-218) antibodyNR5A1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1) (AA 231-280) antibodyNR5A1 适用: 人, 小鼠, 大鼠, 豚鼠, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (2 - 18 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))
- 别名
- NR5A1 / SF1 (NR5A1 产品)
- 别名
- AD4BP antibody, ELP antibody, FTZ1 antibody, FTZF1 antibody, POF7 antibody, SF-1 antibody, SF1 antibody, SPGF8 antibody, SRXY3 antibody, Ad4BP antibody, ELP-3 antibody, Ftz-F1 antibody, Ftzf1 antibody, Ad4BP/SF-1 antibody, Ad4bp antibody, Sf-1 antibody, NR5A1 antibody, sf-1 antibody, elp antibody, sf1 antibody, ftz1 antibody, ad4bp antibody, ftzf1 antibody, SF-1/Ad4BP antibody, SI:zC167P9.2 antibody, ff1d antibody, nr5a2l antibody, nuclear receptor subfamily 5 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1 antibody, nuclear receptor subfamily 5 group A member 1 L homeolog antibody, nuclear receptor subfamily 6 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1b antibody, NR5A1 antibody, Nr5a1 antibody, nr5a1.L antibody, nr5a1 antibody, NR6A1 antibody, nr5a1b antibody
- 背景
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Name/Gene ID: NR5A1
Subfamily: NR5 Fushi tarazu-like
Family: NHR
Synonyms: NR5A1, AD4BP, Adrenal 4 binding protein, Adrenal 4-binding protein, ELP, Steroidogenic factor-1, SRXY3, Steroidogenic factor 1, Nuclear receptor AdBP4, Nuclear receptor elp, POF7, SF-1, SPGF8, Steroid hormone receptor Ad4BP, STF-1, FTZ1, FTZF1, Fushi tarazu factor homolog 1 - 基因ID
- 2516
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location
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