NR0B2 抗体 (Ligand Binding Domain)
-
- 抗原 See all NR0B2 抗体
- NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))
-
抗原表位
- Ligand Binding Domain
-
适用
- 人, 小鼠, 兔, 猴, Hamster
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This NR0B2 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR0B2 Primary Antibody
-
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 1-257) antibody
NR0B2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 110-159) antibodyNR0B2 适用: 人, 兔, 犬, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 1-257) antibodyNR0B2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 1-257) antibodyNR0B2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A11 unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) antibodyNR0B2 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 56-83) antibodyNR0B2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB29166 unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (AA 1-260) antibodyNR0B2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) antibodyNR0B2 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) antibodyNR0B2 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) antibodyNR0B2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: ICC, IF, IHC, IHC-P (4 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))
- 别名
- NR0B2 / SHP (NR0B2 产品)
- 别名
- SHP antibody, SHP1 antibody, SHP-1 antibody, Shp1 antibody, Shp antibody, NR0B2-A antibody, Nr0b2 antibody, SHP-A antibody, gb:bc058069 antibody, nuclear receptor subfamily 0 group B member 2 antibody, nuclear receptor subfamily 0, group B, member 2 antibody, nuclear receptor subfamily 0, group B, member 2a antibody, NR0B2 antibody, Nr0b2 antibody, nr0b2a antibody
- 背景
-
Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR
Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1 - 基因ID
- 8431
- 途径
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
-
