KCNMA1 抗体 (Internal Region)
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- 抗原 See all KCNMA1 抗体
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
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抗原表位
- Internal Region
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适用
- 人, 大鼠, 小鼠, Cow, 斑马鱼, 兔, 马, 非洲爪蟾, 小鸡, 猴, Pig, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KCNMA1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human KCNMA1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from internal region of human KCNMA1. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product KCNMA1 Primary Antibody
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anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (C-Term) antibody
KCNMA1 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 兔, 犬, 马, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1-86) antibodyKCNMA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 124-467) antibodyKCNMA1 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1131-1236) antibodyKCNMA1 适用: 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1080-1129) antibodyKCNMA1 适用: 人, 大鼠, 小鼠, Cow, 兔, 马, 非洲爪蟾, 小鸡, 猴, 绵羊, Pig, 豚鼠, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1097-1196), (C-Term), (Intracellular) antibodyKCNMA1 适用: 人, 大鼠, 小鼠 WB 宿主: 豚鼠 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1184-1200), (C-Term), (Intracellular) antibodyKCNMA1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (Internal Region) antibodyKCNMA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 118-152) antibodyKCNMA1 适用: 人 WB 宿主: 兔 Polyclonal RB53642 unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 641-690) antibodyKCNMA1 适用: 人, 大鼠, 小鼠, Cow, 斑马鱼, 兔, 犬, 马, 猴, 绵羊, Pig, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
- 别名
- KCNMA1 / BK (KCNMA1 产品)
- 别名
- BKTM antibody, KCa1.1 antibody, MaxiK antibody, SAKCA antibody, SLO antibody, SLO-ALPHA antibody, SLO1 antibody, bA205K10.1 antibody, mSLO1 antibody, DDBDRAFT_0190653 antibody, DDBDRAFT_0235401 antibody, DDB_0190653 antibody, DDB_0235401 antibody, KCNMA antibody, KCNMA1 antibody, 5730414M22Rik antibody, BKCa antibody, Slo antibody, Slo1 antibody, mSlo antibody, mSlo1 antibody, KCNMA1b antibody, KCNMA1c antibody, Kcnma antibody, bktm antibody, kcnma1-A antibody, sakca antibody, cSlo antibody, slo1 antibody, kcnma1 antibody, si:ch211-39f22.2 antibody, BcDNA:GH10751 antibody, CG10693 antibody, Dmel\CG10693 antibody, Dslo antibody, dSlo antibody, dSlo1 antibody, dslo antibody, fSlo antibody, potassium calcium-activated channel subfamily M alpha 1 antibody, calcium-activated BK potassium channel, alpha subunit antibody, potassium large conductance calcium-activated channel, subfamily M, alpha member 1 antibody, potassium calcium-activated channel subfamily M alpha 1 L homeolog antibody, potassium large conductance calcium-activated channel, subfamily M, alpha member 1a antibody, calcium-activated potassium channel subunit alpha-1 antibody, slowpoke antibody, KCNMA1 antibody, kcnma1 antibody, Kcnma1 antibody, kcnma1.L antibody, kcnma1a antibody, LOC100454095 antibody, slo antibody
- 背景
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Name/Gene ID: KCNMA1
Subfamily: Potassium channel - calcium-activated
Family: Ion Channel
Synonyms: KCNMA1, BA205K10.1, BKCA alpha subunit, BK channel, BKCA alpha, BKTM, HSlo, K(VCA)alpha, KCa1.1, Maxi-K channel HSLO, MaxiK, KCNMA, SAKCA, SLO-ALPHA, Slowpoke homolog, Stretch-activated Kca channel, Slo homolog, BK channel alpha subunit, Maxi K channel, MSlo, MSLO1, SLO, SLO1 - 基因ID
- 3778
- 途径
- Regulation of Hormone Metabolic Process, Sensory Perception of Sound
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