Endothelin-1 Receptor 抗体 (Extracellular Domain)
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- 抗原 See all Endothelin-1 Receptor (EDNRA) 抗体
- Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))
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抗原表位
- Extracellular Domain
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Endothelin-1 Receptor antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Endothelin A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except EDNRB (72 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Pig (94%) Sheep, Bovine, Bat, Opossum, Platypus, Medaka (89%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from 3rd extracellular domain of human Endothelin A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Horse, Pig (94%), Sheep, Bovine, Bat, Opossum, Platypus, Medaka (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EDNRA Primary Antibody
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anti-Endothelin Receptor Type A (EDNRA) (C-Term) antibody
EDNRA 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (AA 18-80) antibodyEDNRA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A5 unconjugated
anti-Endothelin Receptor Type A (EDNRA) (AA 125-154) antibodyEDNRA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB19282 unconjugated
anti-Endothelin Receptor Type A (EDNRA) (C-Term) antibodyEDNRA 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (AA 413-426), (C-Term), (Intracellular) antibodyEDNRA 适用: 小鼠, 大鼠 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (AA 132-162) antibodyEDNRA 适用: 人 WB, IF, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (C-Term) antibodyEDNRA 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (AA 39-280) antibodyEDNRA 适用: Staphylococcus aureus WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (C-Term) antibodyEDNRA 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Endothelin Receptor Type A (EDNRA) (C-Term) antibodyEDNRA 适用: 人, 小鼠, 猴 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (3 - 16 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))
- 别名
- EDNRA / Endothelin A Receptor (EDNRA 产品)
- 别名
- ET-A antibody, ETA antibody, ETA-R antibody, ETAR antibody, ETRA antibody, hET-AR antibody, ET-AR antibody, Endor antibody, Eta antibody, RGD1559432 antibody, ETa antibody, Gpcr10 antibody, ednr-a antibody, ednra antibody, wu:fb75g12 antibody, ednraa antibody, eta antibody, etar antibody, etra antibody, zgc:85942 antibody, endothelin receptor type A antibody, endothelin receptor type Aa antibody, endothelin receptor type A L homeolog antibody, endothelin receptor type Ab antibody, EDNRA antibody, Ednra antibody, ednraa antibody, ednra.L antibody, ednra antibody, ednrab antibody
- 背景
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Name/Gene ID: EDNRA
Subfamily: Endothelin
Family: GPCR
Synonyms: EDNRA, Endothelin receptor subtype A, Et-a-r, ETRA, Endothelin A receptor, Endothelin eta receptor, Endothelin type a receptor, Endothelin-1 receptor, ETAR, Endothelin receptor type A, HET-AR, Et-a receptor, ETA, ETA-R, Endothelin receptor: subtype a, Endothelin-1-specific receptor, ET-A, ET-RA - 基因ID
- 1909
- 途径
- cAMP Metabolic Process
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