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- 抗原 See all S1PR3 抗体
- S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This S1PR3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human EDG3 / S1P3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TPRX1 (56 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Marmoset, Elephant (94%) Bovine (89%) Gibbon, Rabbit (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from C-terminus of human EDG3 / S1P3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Marmoset, Elephant (94%), Bovine (89%), Gibbon, Rabbit (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product S1PR3 Primary Antibody
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anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 1-378) antibody
S1PR3 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 303-378) antibodyS1PR3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) antibodyS1PR3 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 145-250) antibodyS1PR3 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibodyS1PR3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 201-250) antibodyS1PR3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 302-378) antibodyS1PR3 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibodyS1PR3 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibodyS1PR3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 300-350) antibodyS1PR3 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (4 - 20 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))
- 别名
- SIPR3 / EDG3 / S1P3 (S1PR3 产品)
- 别名
- s1pr3 antibody, si:dkeyp-31e2.1 antibody, EDG-3 antibody, EDG3 antibody, LPB3 antibody, S1P3 antibody, AI132464 antibody, Edg3 antibody, Lpb3 antibody, S1p3 antibody, sphingosine-1-phosphate receptor 3 antibody, sphingosine-1-phosphate receptor 3a antibody, S1PR3 antibody, s1pr3a antibody, S1pr3 antibody
- 背景
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Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3 - 基因ID
- 1903
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