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- 抗原 See all CXCR1 抗体
- CXCR1 (Chemokine (C-X-C Motif) Receptor 1 (CXCR1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CXCR1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CXCR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CXCR2 (50 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee (100%) Gorilla, Orangutan, Gibbon (95%) Monkey (90%) Marmoset, Elephant (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from internal region of human CXCR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee (100%), Gorilla, Orangutan, Gibbon (95%), Monkey (90%), Marmoset, Elephant (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CXCR1 Primary Antibody
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anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (C-Term) antibody
KO Validated CXCR1 适用: 人 IHC (p), ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (AA 186-280) antibodyCXCR1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) antibodyCXCR1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (AA 250-350) antibodyCXCR1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (AA 1-50) antibodyCXCR1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) antibodyCXCR1 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) antibodyCXCR1 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2B8A1 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (N-Term) antibodyCXCR1 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (AA 330-341) antibodyCXCR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 1 (CXCR1) (AA 26-38) antibodyVerified CXCR1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Approved: IHC-P (15 - 20 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15-20 μg/mL. - 说明
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This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
- 有效期
- 12 months
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- 抗原
- CXCR1 (Chemokine (C-X-C Motif) Receptor 1 (CXCR1))
- 别名
- CXCR1 (CXCR1 产品)
- 别名
- C-C antibody, C-C-CKR-1 antibody, CD128 antibody, CD181 antibody, CDw128a antibody, CKR-1 antibody, CMKAR1 antibody, IL8R1 antibody, IL8RA antibody, IL8RBA antibody, Il8ra antibody, CXCR-1 antibody, IL-8RA antibody, CXCR2 antibody, IL8RB antibody, cxcr1 antibody, cxcr2 antibody, C-X-C motif chemokine receptor 1 antibody, chemokine (C-X-C motif) receptor 1 antibody, C-X-C chemokine receptor type 1 antibody, C-X-C motif chemokine receptor 1 S homeolog antibody, CXCR1 antibody, Cxcr1 antibody, LOC100461947 antibody, cxcr1.S antibody
- 背景
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Name/Gene ID: CXCR1
Subfamily: Chemokine
Family: GPCR
Synonyms: CXCR1, C-C, Chemokine c-x-c receptor 1, CKR-1, CMKAR1, CD128, CD181, CD181 antigen, CXCR-1, CXC-R1, IL-8R A, Il8 receptor a, Interleukin 8 receptor a, Interleukin 8 receptor alpha, Interleukin 8 receptor, alpha, Interleukin-8 receptor type A, IL8RBA, Interleukin-8 receptor type 1, C-C-CKR-1, CDw128a, IL-8 receptor type 1, IL8R1, IL8RA - 基因ID
- 3577
- 途径
- cAMP Metabolic Process
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