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- 抗原 See all DPP4 抗体
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
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抗原表位
- Internal Region
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适用
- 人, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DPP4 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CD26. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FAP (56 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%) Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%) Rabbit (88%) Bat, Dog, Cat (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human CD26. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset (100%), Gorilla, Sheep, Elephant, Bovine, Horse, Pig (94%), Rabbit (88%), Bat, Dog, Cat (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product DPP4 Primary Antibody
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anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 484-728) antibody
DPP4 适用: 人 WB, IHC (fro), ELISA, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 631-760) antibodyDPP4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 29-178) antibodyDPP4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (AA 473-588) antibodyDPP4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibodyVerified DPP4 适用: 人 FACS, WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) antibody (PE)DPP4 适用: 人 FACS 宿主: 小鼠 Monoclonal BA5b PE
anti-Dipeptidyl-Peptidase 4 (DPP4) antibodyDPP4 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibodyDPP4 适用: 人, Cow, Pig, 马, 猴, 兔, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) (C-Term) antibodyDPP4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dipeptidyl-Peptidase 4 (DPP4) antibody (FITC)DPP4 适用: 人 FACS 宿主: 小鼠 Monoclonal BA5b FITC
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- 应用备注
- Approved: IHC, IHC-P (1.25 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
- 别名
- DPP4 / CD26 (DPP4 产品)
- 别名
- ADABP antibody, ADCP2 antibody, CD26 antibody, DPPIV antibody, TP103 antibody, MGC81966 antibody, MOP9.8 antibody, MOP9_8 antibody, DPP4 antibody, si:ch73-2d23.3 antibody, Cd26 antibody, Dpp-4 antibody, THAM antibody, dipeptidyl peptidase 4 antibody, dipeptidyl-peptidase 4 S homeolog antibody, prolyl oligopeptidase family protein antibody, dipeptidyl-peptidase 4 antibody, dipeptidylpeptidase 4 antibody, DPP4 antibody, dpp4.S antibody, AT5G24260 antibody, dpp4 antibody, Dpp4 antibody
- 背景
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Name/Gene ID: DPP4
Subfamily: Serine S9B
Family: Protease
Synonyms: DPP4, ADCP-2, ADABP, Dipeptidyl peptidase IV, Dipeptidyl-peptidase 4, DPPIV, ADCP2, T-cell activation antigen CD26, CD26, CD26 antigen, Dipeptidyl peptidase 4, Dipeptidylpeptidase 4, DPP IV, TP103 - 基因ID
- 1803
- 途径
- Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
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