CACNA1C 抗体 (Internal Region)
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- 抗原 See all CACNA1C 抗体
- CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CACNA1C antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CACNA1C / Cav1.2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon (100%) Gorilla, Monkey, Marmoset (94%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human CACNA1C / Cav1.2. Percent identity with other species by BLAST analysis: Human, Gibbon (100%), Gorilla, Monkey, Marmoset (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CACNA1C Primary Antibody
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anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody
CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 unconjugated
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (HRP)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 HRP
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (FITC)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 FITC
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (Biotin)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 Biotin
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (PE)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 PE
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (APC)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 APC
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (PerCP)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 PerCP
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (Atto 488)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 Atto 488
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (Atto 594)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 Atto 594
anti-Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) (AA 1507-1733) antibody (Atto 390)CACNA1C 适用: 兔 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal S57 Atto 390
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- 应用备注
- Approved: IHC, IHC-P (2.5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))
- 别名
- CACNA1C / Cav1.2 (CACNA1C 产品)
- 别名
- CACH2 antibody, CACN2 antibody, CACNL1A1 antibody, CCHL1A1 antibody, CaV1.2 antibody, LQT8 antibody, TS antibody, Cav1.2 antibody, Cchl1a1 antibody, MBC antibody, MELC-CC antibody, RATIVS302 antibody, isl antibody, C-LTCC antibody, CHCACHA1C antibody, ICA antibody, cav1.2 antibody, ca(v)1.2 antibody, CAV1.2 antibody, BCR antibody, rbc antibody, calcium voltage-gated channel subunit alpha1 C antibody, calcium channel, voltage-dependent, L type, alpha 1C subunit antibody, voltage-dependent L-type calcium channel subunit alpha-1C antibody, calcium channel, voltage-dependent, L type, alpha 1C subunit S homeolog antibody, Red blood cell count antibody, RNA, 7SL, cytoplasmic 263, pseudogene antibody, CACNA1C antibody, Cacna1c antibody, cacna1c antibody, LOC100442085 antibody, cacna1c.S antibody, RBC antibody, RN7SL263P antibody
- 背景
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Name/Gene ID: CACNA1C
Subfamily: Calcium channel - alpha subunit Cav1
Family: Ion Channel
Synonyms: CACNA1C, Alpha 1c, CaV1.2, CACH2, CACNL1A1, CCHL1A1, DHPR, alpha-1 subunit, DHP receptor, DHPr, LQT8, TS, CACN2 - 基因ID
- 775
- 途径
- Hormone Transport, Carbohydrate Homeostasis
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