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- 抗原 See all HTR7 抗体
- HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))
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抗原表位
- N-Term
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适用
- 人, 犬, 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HTR7 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
- 品牌
- IHC-plus™
- 特异性
- Human 5HT7 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%) Mouse, Rat, Hamster, Panda (95%) Gorilla, Pig (90%) Horse (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 20 amino acid peptide from N-terminal extracellular domain of human 5HT7 Receptor. Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%), Mouse, Rat, Hamster, Panda (95%), Gorilla, Pig (90%), Horse (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR7 Primary Antibody
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anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (AA 73-84), (Extracellular), (N-Term) antibody
HTR7 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) antibodyHTR7 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (C-Term) antibodyHTR7 适用: 人, 小鼠 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (N-Term) antibodyHTR7 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (AA 1-83) antibodyHTR7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (AA 13-28) antibodyHTR7 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (C-Term) antibodyVerified HTR7 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (AA 47-96) antibodyHTR7 适用: 人, 犬, Pig, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (C-Term) antibodyHTR7 适用: 人 IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))
- 别名
- HTR7 / 5-HT7 Receptor (HTR7 产品)
- 别名
- 5-HT7 antibody, 5-HT-dro antibody, 5-HT-dro1 antibody, 5-HT7Dro antibody, 5-HT[7]Dro antibody, 5-HT[[7Dro]] antibody, 5-HT[[7]] antibody, 5-HT[[7]]Dro antibody, 5-HT[[dro1]] antibody, 5-HTdro1 antibody, 5HT-7 antibody, 5HT-R1 antibody, 5HT-dro antibody, 5HT-dro1 antibody, 5HT[[7Dro]] antibody, 5htdror antibody, CG12073 antibody, DRO1 antibody, Dm5HTdro1 antibody, Dmel\\CG12073 antibody, 5Ht7 antibody, 5HT7 antibody, htr7-A antibody, 5-hydroxytryptamine receptor 7 antibody, 5-hydroxytryptamine (serotonin) receptor 7 antibody, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled antibody, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled L homeolog antibody, HTR7 antibody, 5-HT7 antibody, LOC752003 antibody, Htr7 antibody, htr7.L antibody
- 背景
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Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7 - 基因ID
- 3363
- 途径
- JAK/STAT Signaling
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