兔 anti-山羊 IgG (Heavy & Light Chain) Antibody (Atto 655) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 山羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Atto 655
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-AdsorbedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
- 产品特性
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Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Goat IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.5
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- 应用备注
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Application Note: Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 说明
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Goat IgG ATTO 655 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO655 Conjugation
Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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