我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

AKT1 抗体 (C-Term)

Name: AKT1 抗体 (C-Term)
SKU: ABIN1043730
Availability: OutOfStock

AKT1 适用: 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 14E5-16C8-25F6 unconjugated

Western Blot of Mouse anti-AKT antibody. (AKT1 抗体 (C-Term))

1 image

(4 references)

产品编号 ABIN1043730

Supplier Product No.: 200-301-b26

发货至: 中国

Datasheet as PDF

抗原 See all AKT1 抗体

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
抗原表位
82

45

34

31

28

22

19

18

16

12

10

9

9

8

8

8

8

7

6

6

6

5

5

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
C-Term
适用
480

303

242

54

46

45

33

17

12

11

5

3

3

2

2

1

1

1

1
小鼠
宿主
404

89

7

2

1

1
小鼠
Compatible Secondaries
克隆类型
368

135
单克隆
标记
287

30

22

20

16

15

8

8

8

8

8

8

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1
This AKT1 antibody is un-conjugated
应用范围
397

232

165

78

74

69

50

42

35

31

26

19

15

9

8

6

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Supplier Product No.

200-301-b26

Supplier

Rockland

原理

AKT Antibody

交叉反应（详细）

This antibody is specific for human and mouse AKT protein.

产品特性

Synonyms: mouse anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

纯化方法

This antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

过滤

Sterile filtered

免疫原

Immunogen: Anti-Akt monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human AKT1 protein.
Immunogen Type: Conjugated Peptide

克隆位点

14E5-16C8-25F6

亚型

IgG1 kappa

Top Product

Discover our top product AKT1 Primary Antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibody
AKT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
AKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1F7E10 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1F7G2 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
AKT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 4H12 unconjugated Recombinant Antibody

应用备注

Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 20 μg/mL
Application Note: Anti-AKT monoclonal antibody has been tested by ELISA and western blotting. This antibody is suitable in immunohistochemistry and immunoprecipitation. Expect a band approximately 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:20,000

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Akt Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, (2001) (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, (2001) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, (1987) (PubMed).
抗原

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

别名

AKT1 (AKT1 产品)

背景

Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.

基因ID

207, 62241011

UniProt

P31749

途径

PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect