anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) 抗体

displays differential expression after induction by nerve growth factor [RGD, Feb 2006].. 再加上,我们可以发NEDD8 蛋白 (17)NEDD8 试剂盒 (5)和数多这个蛋白质的别的产品。

列出全部抗体 基因 基因ID UniProt
NEDD8 4738 Q15843
NEDD8 18002 P29595
NEDD8 25490 Q71UE8
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antibodies-online.cn销售最多的anti-NEDD8 抗体

Showing 10 out of 141 products:

产品编号 适用 宿主 标记 应用范围 图像 规格 供应商 交付 价格 详细
Yeast 非结合性 EIA, WB Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody, generated by immunization with full-length recombinant yeast Rub1, was tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # 611-703-127) for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche). Other detection systems will yield similar results. 0.5 mg Log in to see 6至8个工作日
$676.50
详细
非结合性 IHC (p), WB NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). Western blot analysis of NEDD8 expression in rat testis extract ( Lane 1), mouse thymus extract ( Lane 2), mouse brain extract ( Lane 3), HELA whole cell lysates ( Lane 4) and MCF-7 whole cell lysates ( Lane 5). NEDD8 at 9KD was detected using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog #) at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). 100 μg Log in to see 4至6个工作日
$280.00
详细
非结合性 IHC (p), WB Western blot analysis of NEDD8 polyclonal antibody (ABIN388037) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Log in to see 10至11个工作日
$385.00
详细
Cow 非结合性 IP, IHC, WB Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates. 200 μL Log in to see 13至14个工作日
$487.50
详细
小鼠 非结合性 ELISA, FACS, ICC, IHC, WB Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Dilution: 1/10000 Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)  Dilution: 1/500 - 1/2000 100 μL Log in to see 8至11个工作日
$577.50
详细
非结合性 ELISA, ICC, IF, IHC, WB Western blot analysis on Jurkat cell lysate using NEDD8 Antibody,The lane on the left is treated with the antigen-specific peptide. Western blot analysis of extracts from 293, using NEDD8 Antibody. 100 μL Log in to see 11至12个工作日
$390.77
详细
小鼠 非结合性 ELISA, FACS, IF, IHC, WB 100 μg Log in to see 11至14个工作日
$551.83
详细
非结合性 IHC, ELISA, WB Western blot analysis of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2至3个工作日
$302.50
详细
非结合性 EIA, IHC (p), WB 0.4 mL Log in to see 6至8个工作日
$484.00
详细
小鼠 非结合性 CyTOF, ELISA, FACS, ICC, IF, IHC, WB Flow Cytometry: NEDD8 Antibody (5B8) [NBP2-37523] - Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red). Immunocytochemistry/Immunofluorescence: NEDD8 Antibody (5B8) [NBP2-37523] - Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. 0.1 mL Log in to see 7至9个工作日
$516.98
详细

引用最多的anti-NEDD8 抗体

  1. Yeast (Saccharomyces cerevisiae) Polyclonal NEDD8 Primary Antibody for ELISA, WB - ABIN539585 : del Pozo, Estelle: The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1. in Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal NEDD8 Primary Antibody for IHC (p), ELISA - ABIN542757 : Fan, Bigsby, Nephew et al.: The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast ... in Molecular endocrinology (Baltimore, Md.) 2003 (PubMed)
    Show all 3 Pubmed References

  3. Human Monoclonal NEDD8 Primary Antibody for ICC, FACS - ABIN969546 : Ohki, Funatsu, Konishi, Chiba: The mechanism of poly-NEDD8 chain formation in vitro. in Biochemical and biophysical research communications 2009 (PubMed)

更多抗NEDD8的相互作用对抗体

Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Impairment of NEDDylation by Ubc12 knockout enhances PCNA ubiquitination and promotes PCNA-poleta interaction, while up-regulation of NEDDylation by NEDD8 overexpression or NEDP1 deletion reduces the excessive accumulation of ubiquitinated PCNA. Moreover, Ubc12 knockout decreases cell sensitivity to H2O2-induced oxidative stress, but NEDP1 deletion aggravates this sensitivity

  2. NEDD8 has important roles in regulating the progression of bladder cancer (BC) cells and was associated with poor prognosis of patients; hence, it may become a potential therapeutic target of BC.

  3. Data suggest that ubiquitin (Ub) and neural precursor cell expressed developmentally down-regulated 8 (NEDD8) residues 4, 12, 14, and 72 serve as the molecular determinants for specific recognition by ubiquitin specific protease 2 protein (USP2).

  4. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta signaling pathways.

  5. NEDD8-mediated neddylation promotes stress granule assembly.

  6. NEDD8 seems to inhibit the Src-mediated phosphorylation of caveolin-1 by modifying the structure of caveolin-1 protein, which blocks the migration of cancer cells. Although the neddylation process is currently regarded as an emerging target for cancer therapy, our results suggest the possibility that the inhibition of neddylation could facilitate cancer invasion or metastasis at least in some types of cancers.

  7. The cellular effects of NEDD8 inhibition can be manipulated based on the p53 status.

  8. Here we report that high expression of neddylation components, NEDD8 and NAE1, are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence

  9. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  10. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells.

  11. MKK7 undergoes neddylation in human breast cancer cells

  12. complete blocking of CRLs at a higher inhibitor dose-induced cytotoxicity that was amplified by knockdown of CRL regulator Cand1.

  13. In conclusion, the authors revealed that E3 ligase HDM2 promotes NEDD8 NEDDylation of HBx to enhance HBx stability and chromatin localization, which in turn favors HBx-dependent transcriptional regulation, cell proliferation, and hepatitis B virus-driven tumor growth.

  14. CUL5 neddylation may allosterically tune polyubiquitin chain length and topology.

  15. This study identifies MyD88 as a novel substrate of NEDD8, and demonstrate that MyD88 NEDDylation antagonizes its ubiquitination.

  16. This study provides new knowledge of the CFTR biosynthetic pathway. It suggests that SYVN1 and FBXO2 represent two distinct multiprotein complexes that may degrade DeltaF508-CFTR in airway epithelia and identifies a new role for NEDD8 in regulating DeltaF508-CFTR ubiquitination.

  17. Neddylation of Smurf1 activates its ubiquitin ligase activity and Smurf2 exerts Nedd8 ligase activity. This study provided new clues of Smurf2 activation regulation.

  18. Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8.

  19. findings add novel evidence showing, for what we believe the first time, that NEDD8-mediated neddylation is required for normal human endometrial functions

  20. In endothelial dysfunction, HDAC2 levels were reciprocally regulated by ectopic expression of NEDD8 and the de-NEDDylating enzyme SENP8.

Mouse (Murine) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.

  3. neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.

  4. Our findings provide mechanistic evidence of the effects of IAP antagonists in combination with NAE inhibitors, and demonstrate the potential of a new combination therapy for cancer.

  5. a role of Nedd8 in regulating caspase-1 activation following inflammasome activation

  6. Nedd8 and its associated E2 carrier protein (Ubc12) and E3-SCF ligase play a key role in regulating the expression and activity of ENaC in alveolar pneumocytes.

  7. a new regulatory mechanism of RCAN1 function

  8. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TbetaRII at Lys556 and Lys567.

  9. The NEDD8 pathway appears to play an important role in CD4+ T-cell-mediated inflammatory responses

  10. Nedd8 and AhR localize to overlapping areas in the heart and spinal ganglia, raising the possibility that this interaction may play a role in organogenesis

  11. the analysis of expression pattern in the mouse embryo

  12. NEDD8 and ubiquitin function in a redundant manner in controlling receptor endocytic pathways.

  13. SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation

Fruit Fly (Drosophila melanogaster) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. the Cul1-based SCF complex requires Nedd8 modification for the degradation processes of Ci, Arm, and CycE in vivo

  2. Genetic analyses indicate that a balance between neddylation and deneddylation maintained by DEN1 is crucial for animal viability.

NEDD8 抗原简介

蛋白简介

displays differential expression after induction by nerve growth factor

Gene names and symbols associated with NEDD8

  • neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) 抗体
  • neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8) 抗体
  • neural precursor cell expressed, developmentally down-regulated 8 (Nedd8) 抗体
  • CG10679 gene product from transcript CG10679-RB (Nedd8) 抗体
  • neural precursor cell expressed, developmentally down-regulated 8 (nedd8) 抗体
  • Nedd8 (Nedd8) 抗体
  • ubiquitin-like protein Nedd8 (nedd8) 抗体
  • neural precursor cell expressed, developmentally down-regulated 8 L homeolog (nedd8.L) 抗体
  • CG10679 抗体
  • DDBDRAFT_0218116 抗体
  • DDBDRAFT_0238041 抗体
  • DDB_0218116 抗体
  • DDB_0238041 抗体
  • Dmel\\CG10679 抗体
  • dNEDD8 抗体
  • GB17785 抗体
  • NEDD-8 抗体
  • nedd8 抗体
  • nedd8.S 抗体
  • Rub1 抗体
  • si:zc14a17.5 抗体
  • ubiquitin 抗体

Protein level used designations for NEDD8

neddylin , ubiquitin-like protein Nedd8 , NEDD-8 , NEDD8 , neural precursor cell expressed developmentally down-regulated protein 8 , neural precursor cell expressed, developmentally down-regulated gene 8 , CG10679-PA , Nedd8-PA , neural precursor cell-expressed developmentally downregulated , neural precursor cell expressed, developmentally down-regulated 8 , Nedd8 , NEDD8 protein , neural precursor cell expressed, developmentally down-regulated 8 S homeolog

GENE ID SPECIES
4738 Homo sapiens
18002 Mus musculus
25490 Rattus norvegicus
35151 Drosophila melanogaster
286796 Bos taurus
368667 Danio rerio
480265 Canis lupus familiaris
549727 Xenopus (Silurana) tropicalis
552822 Apis mellifera
715763 Macaca mulatta
8621655 Dictyostelium discoideum AX4
100009008 Oryctolagus cuniculus
100301786 Oncorhynchus mykiss
100528101 Ictalurus punctatus
100520086 Sus scrofa
446966 Xenopus laevis
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