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NEDD8 抗体 (Middle Region)

NEDD8 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN4886679
发货至: 中国
  • 抗原 See all NEDD8 抗体
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    抗原表位
    • 15
    • 13
    • 8
    • 3
    • 3
    • 1
    • 1
    AA 20-60, Middle Region
    适用
    • 66
    • 28
    • 24
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 63
    • 4
    • 4
    克隆类型
    • 59
    • 12
    多克隆
    标记
    • 30
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEDD8 antibody is un-conjugated
    应用范围
    • 38
    • 27
    • 27
    • 13
    • 13
    • 12
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for NEDD8(NEDD8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    序列
    TDKVERIKER VEEKEGIPPQ QQRLIYSGKQ MNDEKTAADY K
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for NEDD8(NEDD8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: neural precursor cell expressed, developmentally down-regulated 8
    Protein Name: NEDD8
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence in the middle region of human NEDD8 (20-60aa TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK), identical to the related mouse and rat sequences.
    亚型
    IgG
    Top Product
    Discover our top product NEDD8 Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • 抗原
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    别名
    NEDD8 (NEDD8 产品)
    别名
    NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody
    背景
    NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60 % amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

    Synonyms: NED8 | NEDD 8 | NEDD-8 | Nedd8 | Neddylin | Rub1 | Q15843
    基因ID
    4738
    UniProt
    Q15843
    途径
    Ubiquitin Proteasome Pathway
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