Granzyme B (GZMB) (AA 17-247) (Active) 蛋白

ABIN2666524 产品详细信息, 供应商: Log in to see
蛋白名称
产品特性
AA 17-247
4
2
2
2
2
1
1
1
1
1
1
1
宿主
小鼠
15
13
1
1
1
资源
HEK-293
7
7
4
3
2
2
1
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Flow Cytometry (FACS)
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纯度 > 95 % , as determined by Coomassie stained SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平

Less than 1.0 EU per μg of protein as determine by the LAL method.

背景 Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumorous cells. This is evident by the fact that CTLs from mice deficient of granzyme B exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T cells induces substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardia infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.
分子量 This 246 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kDa. The protein migrates at approximately 37 kDa in DTT-reducing conditions and approximately 37 kDa in non-reducing conditions by SDS-PAGE. The predicted N-term
研究领域 Immunology, Innate Immunity, Adaptive Immunity, Apoptosis/Necrosis, Inflammation, Enzymes, Metabolism
应用备注 Optimal working dilution should be determined by the investigator.
说明

Biological activity: Recombinant mouse Granzyme B supplied in its inactive form. Once the mouse Granzyme B is activated by active mouse Capthepsin C/DPPI, it is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-ThioBenzyl ester (Boc-AAD-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB)1,2, with an activity > 3000 pmol/min/μg.

限制 仅限研究用
状态 Liquid
溶解方式 For maximum results, quick spin vial prior to opening. The recombinant protein could be aliquoted and stored at -20 °C in a sterile buffer (20 mM Tris, 150 mM NaCl, and pH 7.5).
浓度 200 μg/mL
缓冲液 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.5.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 -20 °C
储存方法 Unopened vial can be stored at -70°C for six months.
厂商提供的图像
ELISA image for Granzyme B (GZMB) (AA 17-247) (Active) 蛋白 (ABIN2666524) Granzyme B (GZMB) (AA 17-247) (Active) protein
背景 Hiebert, Granville: "Granzyme B in injury, inflammation, and repair." in: Trends in molecular medicine, Vol. 18, Issue 12, pp. 732-41, 2012 (PubMed).

Saito, Kondo, Hojo: "Granzyme B as a novel factor involved in cardiovascular diseases." in: Journal of cardiology, Vol. 57, Issue 2, pp. 141-7, 2011 (PubMed).

Grossman, Verbsky, Tollefsen, Kemper, Atkinson, Ley: "Differential expression of granzymes A and B in human cytotoxic lymphocyte subsets and T regulatory cells." in: Blood, Vol. 104, Issue 9, pp. 2840-8, 2004 (PubMed).

Edwards, Kam, Powers, Trapani: "The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes." in: The Journal of biological chemistry, Vol. 274, Issue 43, pp. 30468-73, 1999 (PubMed).

Smyth, McGuire, Thia: "Expression of recombinant human granzyme B. A processing and activation role for dipeptidyl peptidase I." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 12, pp. 6299-305, 1995 (PubMed).

Heusel, Wesselschmidt, Shresta, Russell, Ley: "Cytotoxic lymphocytes require granzyme B for the rapid induction of DNA fragmentation and apoptosis in allogeneic target cells." in: Cell, Vol. 76, Issue 6, pp. 977-87, 1994 (PubMed).

Trapani, Klein, White, Dupont: "Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 18, pp. 6924-8, 1988 (PubMed).

Schmid, Weissmann: "Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 1, pp. 250-6, 1987 (PubMed).