HIF1A 抗体 (AA 610-727)
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- 抗原 See all HIF1A 抗体
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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抗原表位
- AA 610-727
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HIF1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human HIF-1alpha aa. 610-727
- 克隆位点
- 54-HIF
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HIF1A Primary Antibody
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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Effects of cellular iron deficiency on the formation of vascular endothelial growth factor and angiogenesis. Iron deficiency and angiogenesis." in: Cancer cell international, Vol. 10, pp. 28, (2010) (PubMed).
: "Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15162-70, (2002) (PubMed).
: "Endoglin expression is regulated by transcriptional cooperation between the hypoxia and transforming growth factor-beta pathways." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43799-808, (2002) (PubMed).
: "Constitutive activation of hypoxia-inducible genes related to overexpression of hypoxia-inducible factor-1alpha in clear cell renal carcinomas." in: Cancer research, Vol. 61, Issue 13, pp. 5215-22, (2001) (PubMed).
: "Oxygen sensing and HIF-1 activation does not require an active mitochondrial respiratory chain electron-transfer pathway." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21995-8, (2001) (PubMed).
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Effects of cellular iron deficiency on the formation of vascular endothelial growth factor and angiogenesis. Iron deficiency and angiogenesis." in: Cancer cell international, Vol. 10, pp. 28, (2010) (PubMed).
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- 抗原
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- 别名
- HIF-1alpha (HIF1A 产品)
- 别名
- hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody
- 背景
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Molecular oxygen (O2) is essential for mammalian metabolic processes such as oxidative phosphorylation. Thus, survival depends upon instantaneous transcriptional modulation of genes that maintain O2 homeostasis. Transcriptional control of several of these genes is mediated by hypoxia-inducible factor 1 (HIF-1). HIF-1 is a heterodimer whose alpha and beta subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. Common structural features of these proteins are an N-terminal bHLH DNA-binding domain and multiple PAS domains that confer dimerization ability and target gene specificity. Members diverge in their C-terminal regions. HIF-1beta is also known as the arylhydrocarbon nuclear translocator which is part of the functional dioxin receptor. However, HIF-1alpha functions exclusively to mediate responses to O2 deprivation. It contains C-terminal and internal transactivation domains. Although HIF-1 aproteinlevels increase during hypoxia, it is unstable in the presence of O2 due to an oxygen-dependent degradation domain (ODD) that targets it for ubiquitination. Thus, HIF-1alpha is essential for functional HIF-1 to mediate gene transcription in response to hypoxia.
Synonyms: MOP1 - 分子量
- 120 kDa
- 途径
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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