MAP2K5 抗体 (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430)

Details for Product anti-MAP2K5 Antibody No. ABIN754183, 供应商: Log in to see
抗原
  • Mek5
  • HsT17454
  • MAPKK5
  • MEK5
  • PRKMK5
  • AI324775
  • AI428457
  • Mapkk5
  • Prkmk5
  • fb73f02
  • wu:fb73f02
  • zgc:172137
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAP2K_A
  • mitogen activated protein kinase kinase 5
  • mitogen-activated protein kinase kinase 5
  • Map2k5
  • MAP2K5
  • map2k5
  • MKK5
Alternatives
anti-大鼠 MAP2K5 抗体 for ELISA
抗原表位
AA 380-430
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30
15
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14
14
11
11
9
6
6
6
5
4
4
3
2
2
2
2
1
1
1
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1
适用
人, 小鼠, 大鼠
168
88
81
11
7
7
6
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3
3
3
2
2
2
2
1
1
宿主

162
16
克隆类型
多克隆
标记
This MAP2K5 antibody is un-conjugated
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9
9
5
5
5
5
5
5
5
5
5
5
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4
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
110
63
47
28
24
22
4
3
2
1
1
1
Supplier
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Supplier Product No.
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Available images

'独立验证'标志
Lot Number 120828
Method validated Immunohistochemistry
Positive Control Human breast myoepithelial and glandular tissue
Negative Control Human breast adipose tissue
Notes Strong signal was detected in positive control sample, with minor background seen in negative control sample.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)
  • Catalog number: ABIN754183
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  • Supplier catalog number: Log in to see
  • Lot number: 120828
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
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  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human breast myoepithelial and glandular tissue stained with antibody
  • Negative control: Human breast adipose tissue stained with antibody
  • Isotype control: Human breast myoepithelial and glandular tissue stained with isotype control
  • Secondary only control: Human breast myoepithelial and glandular tissue stained with secondary antibody only
实验流程
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 1: Human breast tissue stained with anti-MAP2K5 (brown) and counterstained wit...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 2: Human breast adipose tissue stained with anti-MAP2K5 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 3: Human breast tissue stained with isotype control antibody (brown) and count...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 380-430) antibody (ABIN754183) Figure 4: Human breast tissue stained with secondary antibody only (brown) and counte...
免疫原 KLH conjugated synthetic peptide derived from human MEK5
亚型 IgG
纯化方法 Purified by Protein A.
别名 Mek5 (MAP2K5 Antibody 摘要)
背景

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Subcellular location: Cytoplasm, Nucleus

Synonyms: MEK5, MAPKK5, Dual specificity mitogen-activated protein kinase kinase 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MAP kinase kinase 5

基因ID 5607
UniProt Q13163
研究领域 Cancer, Kinases/Phosphatases
途径 MAPK Pathway, Neurotrophin Signaling Pathway
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-MAP2K5 抗体 (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 P...
Immunohistochemistry (IHC) image for anti-MAP2K5 抗体 (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-MAP2K5 抗体 (Mitogen-Activated Protein Kinase Kinase 5) (AA 380-430) (ABIN754183) Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 fo...
有引用在: Chen, Hou, Ye, Chen, Cui, Tian, Li, Liu: "MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling." in: Scientific reports, Vol. 4, pp. 3819, 2014 (PubMed).

Li, Dong, Wei, Wang, Zhang, Li: "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." in: International journal of biological sciences, Vol. 10, Issue 2, pp. 171-80, 2014 (PubMed).